SpectraMax Spectrofluorometers. 2. SpectraMax M2 and SpectraMax M2 e readers can acquire absorbance as well as fluorescence data for samples by issu ing a single read command. SpectraMax® ABS Plus readers use a grating monochromator to select the exact wavelength needed for every assay. e. It can run samples based on pre-defined. Set up template in SoftMax Pro and read plate on the SpectraMax M5 microplate reader: Note: IMAP FP protocols for use with FAM- and TAMRA-labeled substrates are available in SoftMax Pro 5 in the Binding Assays protocol folder. com. For a complete list of the instruments supported by this release of the SoftMax Pro Software, see Supported Instruments on page 111. SoftMax Pro Software was used to analyze data, and the net ΔRFU values calculated were used to plot a standard curve, generate the R 2 value, and show the sensitivity of this reader in detecting very low endotoxin levels. \ Figure 3. 20 μL of Component A was added to each well of the microplate. More Product Information. Unlike conventional plate readers, multimode microplate readers are able to perform two or more forms of. These instruments often feature additional detection modes in addition to fluorescence intensity, such as UV-vis absorbance, fluorescence. Plate chamber design of the SpectraMax category 1 microplate readers. Microplate readers use a vertical light path so the distance of the light through the sample depends on the volume. . for the SpectraMax Multi-Mode Microplate Reader, as well as all other Molecular Devices' microplate readers. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. Because microplate readers are meant to shoulder routine and time-consuming tasks for researchers, ease of use is an important feature. In SOFTmax PRO, set up a plate section for an excitation scan with no cutoff filter and use the medium PMT setting. SpectraMax ® M2 Multi-Mode Microplate Reader. Table 1. 470 nm). Supported Plates Microplates having 6, 12, 24, 48, 96, and 384 wells can be used in the SpectraMax Multi-Mode Microplate Readers. 313 reference gain check fail. It also integrates with the. 10 µL of BSA standard or cell lysate was pipetted into wells of a 96-well microplate (n = 3). The SpectraMax® ABS Plus Microplate Reader can run both cuvette-based and microplate reader applications on the same instrument. IMAP FP KINASE ASSAYS ON THE SPECTRAMAX M5 MULTI-MODE MICROPLATE READER 校准标准品的制备 Table 1. The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. With three modes of detection for UV-Vis absorbance, fluorescence and luminescence, the SpectraMax Mini reader is user upgradeable - from 2 to 3 modes, providing researchers with a versatile. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. The SpectraMax iD3 reader is a stand-alone multi-mode microplate reader that introduces NFC based user authentication and touchscreen interface personalization. begin reading microplates with seven mouse clicks. The SpectraMax® Paradigm Multi- Mode Microplate Reader with Tunable Wavelength (TUNE) Detection Cartridge from Molecular Devices combines the flexibility of wavelength scanning with the high sensitivity of filters. With three modes of detection for UV-Vis absorbance, fluorescence and luminescence, the SpectraMax Mini reader is user upgradeable - from 2 to 3 modes, providing researchers with a versatile instrument that. From the Windows Start menu, go to All programs > Molecular Devices > SoftMax Pro x. Our extensive SpectraMax® line of user-friendly microplate readers – the industry’s most cited brand – provide great flexibility for labs conducting applications ranging from ELISAs to nucleic acid and protein quantitation, and include absorbance, fluorescence, luminescence and more. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). This version is compatible with the following instruments: SpectraMax® Mini Multi-Mode Microplate Reader (software version 7. 58% for the detection module and 2. The SpectraMax L reader is available in 1- and 2-channel (Photomultiplier Tube) configurations. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change filters. 2 only: SpectraMax Mini Update. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. Examples: “Press carefully to engage,” “ Do not press down. 1 software Download page. 专为科研检测平台量身打造的灵活且强大的小型微孔板读板机（酶标仪），具备终点法和动力学检测模式，标准配置6块滤光片，覆盖蛋白定量、细胞活力、农药残留、ELISA实验等. Other features and benefits include: Eliminate changing out filters. Read More. When the incubator is on, the temperature may be controlled from 2°C above ambient to 60°C. Page 35: Setting Up The Instrument SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Setting up the Instrument 1. Download PDF. The instrument is designed to regulate the temperature of the plate chamber from 5°C above ambient to 45°C. The AlphaScreen Detection Cartridges utilize a 680nm Laser Diode to provide a sensitive reading system for AlphaScreen assays. . For fluorescence intensity, time resolved fluorescence, and fluorescence polarization assays, the SpectraMax M5 Microplate Reader optical design provides the highest level of flexibility. For biological applications involving small samples, such as purificationThe SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. This application note describes experimental protocols that enable the detection of relatively low numbers of cells expressing Green Fluorescent Protein. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. The SpectraMax® iD3 and iD5 measure absorbance, fluorescence, and luminescence. Preparation of Calibration Standards Calibration Standard (% Phosphorylated) µL Peptide Stock µL Phospho-peptide Stock 0 100 0 12. Using the optimized wavelength settings, the SPECTRAmax GEMINI XS fluorescence microplate reader can distinguish between live and dead cells using this assay. . 2. Table of Contents. Open the Protocol Library, locate the ‘Protein Quant’ folder, and open the pre-configured protocol. 2) Add in your protocol in Method Editor the Fluorescence Polarization strip. Wide range of applications. Measuring platelet aggregation with a microplate reader. SpectraMax Mini Plug-In for SoftMax Pro 7. Add to Cart. Italic and boldface type are used for emphasis. On the back, in the right hand top corner,. 3) This warranty. Fluorescence Microplate Reader Comparison Chart. 0 out of 5The SpectraMax ® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. This tunable visible absorbance reader is for 96- and 384-well microplates and acquires data in endpoint, kinetic, and spectral scan modes using wavelengths from 340-850 nm, tunable in 1 nm increments. The introduction of the Molecular Devices’ SPECTRAmax PLUS microplate spectrophotometer marks the first time that photometric measurements made in a microplate format can be automatically normalized such that the resultant values are identical to those obtained with the corresponding solutions in a standard 1 cm. Download PDF. The SpectraMax® M2 and M2e Multimode Microplate Readers provide two detection modes in one platform and offer the added functionality of bottom-read fluorescence for cell-based assays. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. Open the top cover of the instrument. The high sensitivity, low-noise photomultiplier tubes (PMTs), and variable-volume. Instrument design, user interface capabilities, and cost play key roles in purchasing a suitable microplate reader. Microplate Readers Molecular Devices Spectramax M5 Microplate Reader. Nucleic Acid and Protein Quantitation. degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner. 305 reference level saturation. Learn about the features, specifications and applications of SpectraMax M2/M2e Microplate Readers, a multi-detection system with dual-mode cuvette ports and dual monochromators. The data sheet provides detailed information on the system's optical performance, data collection and analysis, plate stacker and robot integration, and validation options. Category 2 and 3 microplate readers use gradient heating as well. The SpectraMax 190 reader can be integrated with Molecular . The SpectraMax i3x Multi-mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with added functaionlity of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. Like conventional microplate readers, multimode microplate readers detect reaction signals from microtiter plates in order to perform assays such as ELISA, protein detection, and drug screening assays. Products : SpectraMax 250 (Obsolete) SpectraMax® 250 User Manual Download Page. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. It measures the optical density (OD) of samples in both 96- and 384-well. The SpectraMax i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with user-configurable detection modules for western blot, cell imaging, and fast kinetics. Every SpectraMax® 340 PC 384 microplate reader has an optical system built around a monochromator. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. The dual monochromator optics allow. M2. Microplate readers’ standard detection modes. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. For more sample throughput, both readersThe SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry-leading SoftMax Pro. It was also read on the monochromator-based. The data acquisition and analysis is easily streamlined when using the preconfigured protocol in Softmax Pro Sofware. Next generation SpectraMax® absorbance microplate readers. SpectraMax 340PC384 Microplate Reader A versatile 96- and 384-well format absorbance reader let the monochromator tune to that exact wavelength. Use similar settings for other Molecular Devices readers with fluorescence detection mode. begin reading microplates with seven mouse clicks. ATP standard curves read on SpectraMax microplate readers. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. The SpectraMax® L Microplate Reader measures flash and glow luminescence assays in both 96- and 384-well plates. B: Selection of Excitation Wavelength. 0112-0115J 9 Chapter1:Introduction TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-ModeMicroplate Data Acquisition and Analysis Software, users are now able to extend the ease-of-use of their typical plate reader applications to cell-based imaging and western blot detection. SpectraMax and VersaMax Plate Readers OperatorÕs Manual Ñ 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. The SPECTRAmax™ PLUS 384 microplate spectrophotometer provides rapid and sensitive UV/VIS measurements of a variety of analytes including specific proteins, nucleic acids, and other molecules across a wide range of concentrations. SpectraMax 250 User. This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. 230-1000 nm, Monochromator, ABS, FLR (top and bottom) and LUM, with incubation. Page 22 SpectraMax iD3 Multi-Mode Microplate Reader User Guide 2. unconventional microplate reader assays, including imaging cytometry and western blots • Protocol sharing with a strong user community • Spectral optimization wizard to maximize assay signal window (SpectraMax i3 and Paradigm readers only) SoftMax Pro Software SoftMax Pro® Software is the most publishedi3x. SpectraMax M2 and SpectraMax M2e readers can acquire absorbance as well as fluorescence data for samples by issuing a single read command. Eight differentSpectraMaxi3xMulti-ModeDetectionPlatformUserGuide 4 5039228D Chapter6:SpectraMax InjectorCartridge 79 InstalltheInjectorCartridge 79 UsingtheInjectorCartridge 86The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget friendly solution that gives you exactly what you need when you need it. The SpectraMax® ABS Plus Microplate Reader can run . no. Italic and boldface type are used for emphasis. The SpectraMax M5e Micoplate Reader is the standard for UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-well and 384-well microplates. The SpectraMax® M3 Multi-Mode Microplate Reader dual monochromatic optics allows the widest range of applications to be utilized for bioresearch applications. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). Find our technical support information along with contact details. The SpectraMax Injector System with SmartInject™ Technology features low dead volume (10 pL), overflow protection, and ensures. This pathlength correction is accomplished only with the use of. These lab workhorses are used in drug discovery, research, bioassay. Title: SpectraMax i Series Multi-Mode Microplate Readers | Molecular Devices Author: Molecular Devices Subject: SpectraMax i Series offer the ideal solution for any application whether you are looking for an intuitive, easy-to-use reader, multi-user lab, or an upgradeable system that can adapt to support your lab s new projects. 305 reference level saturation. 5 25 75 25 50 50 50 100 0 100 结合反应 Table 2. An enhancer is provided in the kit where applicable to further reduce background fluorescence and to increase the. The SpectraMax® M2 and M2e Multimode Microplate Readers provide two detection modes in one platform and offer the added functionality of bottom-read fluorescence for cell-based assays. User guide for SpectraMax M5 Microplate Reader Before beginning, you should contact Tatiana Laremore ([email protected], high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. 1. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. For biological applications involving small samples,Minimum volume and maximum throughput with 96- or 384-well microplate compatibility. Most papers they cultured bacteria to OD600>0. SpectraMax® Plus 384 provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. Introduction. Detailed specifications of each model reader are reported in Section 7, “Specifications”. Expanded read mode capabilities Equipped for high performance, the SpectraMax iD5. com 4. SpectraMax® Microplate Readers and SoftMax® Pro Software. Assays can be better optimized by scanning across. Download the SpectraMax Mini Reader Plug-in and associated guides. Preparation of Calibration Standards Calibration Standard (% Phosphorylated) µL Peptide Stock µL Phospho-peptide Stock 0 100 0 12. Intended Use/Discipline:Biological. Figure 1. Molecular Devices: Molecular Devices. 0112-0115 F 7 Description 1 Introduction The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate readingSpectraMax i3x. 0 mm Holex HEXAGON ballhead bolt driver to loosen the screw located inside the Transport Lock opening until you feel the spring release. Microplate readers use a vertical light path so the distance of the light through the sample depends on the volume. The eight-channel system- consisting of 8 sample beams and 8 reference beams delivers both superior. zip. The SpectraMax iD3 reader is built on the same foundation that has made our entire SpectraMax microplate reader product line among the most trusted in the industry. Guides: SpectraMax Mini Installation Guide. The SpectraMax® i3 is Molecular Devices latest multi-mode microplate reader which delivers several applications including; Ultra-Violet to visible absorbance detection, fluorescence intensity and multi-colour luminescence. The 4 injector configuration enables flash and glow BRET assays and a 2-fold improvement in speed for traditional flash and glow assays. The FlexStation® 3 Multi-Mode Microplate Reader measures absorbance, fluorescence intensity, fluorescence polarization, luminescence, and time-resolved fluorescence. The Molecular Devices SpectraMax M5 is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. 0 out of 5 $ 5,999. The following detection cartridge is required: • Fluorescence Polarization (FP-FLUO) Detection Cartridge. : 2 Oct 12 Page 6 of 20 Setup Guide on the Molecular Devices SpectraMax® Paradigm® Microplate Detection Platform Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. Detection modalities include absorbance (UV-Vis Abs), fluorescence intensity (FI), luminescence (Lumi), and all have. The temperature of the microplate chamber can be regulated, if desired from 4°C above ambient to 45°C. Type: Clear: Add to Cart. With optimized reagents and the industry-leading data acquisition andSpectraMax® Plus 384 microplate reader can run both standard spectrophotometer and microplate reader applications on the same instrument. The optimized settings listed in Table 1 can be transferred to any plate reader of the same model, i. HTRF-compatible SpectraMax Multi-Mode Readers. The analysis capabilities of the software provide quantitation in an easy-to-read, user customizable report format. Blank subtraction and standard curve fitting were done automatically by the software. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. The Molecular Devices SpectraMax XPS has an optical design that is the only multi-channel system that truly mimics a dual-beam spectrophotometer. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. 303 unable to cal dark current. Selection of Water Constant in PathCheck settings. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. The 2-channel configuration is available with 0, 2, or 4 injectors. Page 28 Chapter 5: Read Modes and Read Types 1. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. Expanded read mode capabilities Equipped for high performance, the SpectraMax iD5 is HTRF®-certified, DLReady, and SpectraMax® DuoLuc™certified. The fluorometric performance of the SpectraMax M2e is similar, but slightly superior to that of the Gemini EM, a dedicated top and bottom read fluorescence microplate reader. Learn how to operate the VersaMax, SpectraMax 340PC384 and SpectraMax 190 Plus384 microplate readers, which offer fast and accurate absorbance measurements for a variety of assays. The SpectraDrop Microplate incorporates a specially designed adapter and a slide pair whose optical clarity allows measurements in absorbance and. The SpectraMax Multi-Mode Microplate Readers are packed in a specially designed carton. Page 1 SpectraMax M2/M2 user guide ® A MULTI-DETECTION MICROPLATE READER WITH TWO-MODE CUVETTE PORT. Which SpectraMax M Series reader do you need? SpectraMax M Series Multi-Mode Microplate Readers 5 Ordering information VWR cat. The SpectraMax® ABS Plus Microplate Reader can run . 3) At the bottom of the strip, click on "Show. Microplate automation and detection instruments for walk away operation and high quality results. For SpectraMax ABS Plus reader, r 2 = 0. Page 38 SpectraMax iD3 Multi-Mode Microplate Reader User Guide Bottle Holder The bottle holder is mounted on two knobs for ease of installation, cleaning, and replacement. , a leading provider of high-performance life science solutions and a company of Danaher Corporation (NYSE:DHR), has launched its SpectraMax® Mini Multi-Mode Microplate. Learn about the SpectraMax i3x Multi-Mode Plate Reader capabilities and options in this short video. flexibility of monochromator-based systems. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. The SpectraMax® M3 Multi-Mode Microplate Reader is a UV/visible reader with dual monochromatic optics and three modes of detection for bioresearch procedures. 316 grating motor driver faulty. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. Example: [Drawer]. The guide also covers the software features, the specifications, and the accessories of the systems. Endpoint, kinetic, spectrum. 0112-0115 F 7 Description 1 Introduction The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate readingevery Gemini EM Reader. Align the two slots on the bottle holder with the two pegs on the rail. edu) to be added as a trained user in iLab. both cuvette-based and microplate reader applications on the same instrument. SpectraMax Plus: SpectraMax Plus. With optimized reagents and the industry-leading data acquisition and analysistool, SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to perfectly. In this application note, we demonstrate how to measure bacterial cell growth via absorbance at 600 nm (reported as OD 600) over the course of 24 hours using a discontinuous kinetic reading protocol on the SpectraMax ® iD3 Multi-Mode Microplate Reader. Title: SpectraMax i Series Multi-Mode Microplate Readers | Molecular Devices Author: Molecular Devices Subject: SpectraMax i Series offer the ideal solution for any application whether you are looking for an intuitive, easy-to-use reader, multi-user lab, or an upgradeable system that can adapt to support your lab s new projects. The SpectraMax® ABS and ABS Plus absorbance microplate readers provide the flexibility, sensitivity, and convenience for a wide range of assays such as ELISAs, microbial growth, and protein quantitation. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. 1) Select the correct plate and the wells that you want to measure. Examples: “Press carefully to engage,” “ Do not. SoftMax® Pro 7. SpectraMax Quant dsDNA Assay Kits are highly selective for dsDNA. Also review the Maintenance and Troubleshooting chapter of the user guide for your model plate reader. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry leading SoftMax Pro. Figure 1: Endotoxin standard curves on SpectraMax microplate readers, plotted using a log-log curve fit. Read one sample or up to 384 inThe SpectraMax® ABS Microplate Reader bridges the gap between the affordability of filter-based readers and the . SpectraMax Paradigm Microplate Reader General specifications User-upgradeable Multi-Mode Detection Platform Technical specifications Optimized Guaranteed* Fluorescence Intensity (FI) (Fluorescein) 96-well (200 µL) 0. Temperature in the microplate chamber is isothermal, both at ambient and when the incubator is turned on. Lift up the lid over the chamber to insert or remove a cuvette. The Molecular Devices SpectraMAX absorbance microplate readers mimic a dual beam spectrophotometer with a patented multichannel optical design. An ATP standard curve spanning five decades was run using the ATPlite 1step Luminescence Assay System (PerkinElmer) on the SpectraMax iD3 reader. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. Detection modalities include absorbance (UV-Vis Abs) and. Use the provided 3. Products : General, Microplate Readers. Lower limits of detection for ATP determined using the ATPlitePage 1 ® SPECTRAmax Microplate Spectrophotometer Operator’s Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0085 Rev. Chapter5:ReadModesandReadTypes 0112-0115J 27 Microplate Data Acquisition and Analysis Software, users are now able to extend the ease-of-use of their typical plate reader applications to cell-based imaging and western blot detection. The SpectraMax® ABS Plus Microplate Reader can run . 1. The SpectraMax M2 and M2e readers can be integrated with the StakMax® Microplate Stacker from Molecular Devices in a matter of minutes. A SpectraMax M2 microplate reader helps minimize operating time and save costs, allowing researchers to spend more time analyzing data and generating practical knowledge. The dual monochromator optics allow the widest range of. The dual monochromator optics allow the. Contents of wells were pipetted up and down several times to mix. Brochure. Cell Analysis. Examples: “Press carefully to engage,” “ Do not. Sensitive fluorescent quantitation of DNA with the Quant-iT. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). Programmable liquid handling allows for perfectly timed, automated biochemical and cell-based assays. The SpectraMax 190 reader can be integrated with Molecular . 0 out of 5 $ 5,999. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. 5 87. Our SpectraTest® ABS2, FL1, LM1, SpectraMaxi3xMulti-ModeDetectionPlatformUserGuide 4 5039228D Chapter6:SpectraMax InjectorCartridge 79 InstalltheInjectorCartridge 79 UsingtheInjectorCartridge 86 The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget friendly solution that gives you exactly what you need when you need it. This system includes the ScanLater Western Blot Detection Cartridge, ScanLater Western Blot Kit and image acquisition software powered by SoftMax Pro® Software. Chapter 1: Introduction The SpectraMax® Mini Multi-Mode Microplate Reader is a multi-detection instrument with a 6- well to 384-well plate read capability. SpectraMax i3x Multi-Mode Detection Platform. Gemini XPS Microplate Reader (or other SpectraMax reader with fluorescence detection mode) SoftMax Pro Software; Methods Set up the instrument and software. If you do not have an iLab account you must first make one: Spectramax 190 absorbance microplate reader utilizes a patented multichannel optical design to simulate a dual beam spectrophotometer to help eliminate errors through the usage of a dual fiber optic exposure. The plate reader can measure multiple readouts, including UV and visible absorbance. Molecular Devices. Create custom fluorescence assays without having to purchase expensive filter sets. The SpectraMax ® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. The calculated LOQ values were 0. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. SpectraMax iD5 Multi-Mode Microplate Reader Optional, for SpectraMax iD5 reader: Fluorescence Filter with holder 565nm BW 30nm (Molecular Devices cat. Or scan up to 384 wells at any time to determine the best assay conditions. For more sample throughput, the system can SpectraMax iD5; SpectraMax Mini; SpectraMax Mini Install Guide; SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. 6% for the on-board optics (monochromators). #6590-0131) Fluorescence Filter with holder 625nm BW 35nm (Molecular Devices cat. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. SpectraMax® Microplate Reader Suite of Products Knowledge Base Published Dec 16, 2020 | Updated Dec 06, 2021 Products : SpectraMax i3x reader,. for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. The SpectraMax® M2 and M2e Systems from Molecular Devices are multidetection microplate readers with dualmonochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). SpectraMax® Gemini™ EM Microplate Reader, Molecular Devices. The SpectraMax M2e is a dual-monochromator, multi-detection microplate reader with a dual-mode cuvette port and 96 or 384 microplate reading capability. The reader can be equipped with a NanoBRET detection for a 4-fold improvement in limit of. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. Highly customizable low volume. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideMinimum volume and maximum throughput with 96- or 384-well microplate compatibility. With optimized reagents and the industry-leading data acquisition and analysis tool, SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow toFlexStation® 3 Multi-Mode Microplate Reader. Furthermore, VersaMax system is reliable, which means more uptime and lower long-term cost of ownership. The dual monochromator optics allow. We would like to show you a description here but the site won’t allow us. View your data quickly using the large touchscreen interface, export your data for analysis in the program of. Table of Contents. microplate reader. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Figure 1. The Gemini XPS Reader can be integrated with Molecular Devices’ StakMax ® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. Total volume (except deep. Spectranet customer portalWe would like to show you a description here but the site won’t allow us. Read one sample or up to 384 in0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. SpectraMax® M2 Multi-Mode Microplate Reader MLDVM2 SpectraMax® M2e Multi-Mode Microplate Reader (top and bottom reading) MLDVM2E SpectraMax® M3 Multi-Mode Microplate Reader MLDVM3 Ordering information VWR cat. This unlocks the transport slide. The SpectraMax® M3 Multi-Mode Microplate Reader dual monochromatic optics allows the widest range of applications to be utilized for bioresearch applications all without the need to change filters, and provides three modes of detection in one platform. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. The SpectraMax Plus 384 reader is equipped with the innovative technology patented by Molecular Devices, the PathCheck® Sensor which measures the depth of samples in a microplate through optical path. SpectraMax iD5 Multi-Mode Microplate Reader. The plate was placed on an shaker for 15 seconds and then incubated for 2 hours at 37°C, 5% CO 2. The SpectraMax® i3x reader allows you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one system. 72 MB. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time. Find regional support. For the calculation of the ratiometric biosensor signal, the emission intensity at 510 nm was recorded upon an excitation at 380 nm and 470 nm and the former was divided by the latter (Exc. The QuickSync client and automatic USB export option not only frees the user from waiting for data in front of the plate reader, but automatically sends it to one’s computer in an. The SpectraMax® is a compact and economical solution designed to save researchers benchtop space and budget. We would like to show you a description here but the site won’t allow us. SPECTRAmax PLUS Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS. , a leading provider of high-performance life science solutions and a company of Danaher Corporation (NYSE:DHR), has today launched its. , a. Microplate spectrophotometers, also called microplate readers, allow researchers to analyze multiple samples in parallel using 6- to 1536-well formats, as opposed to single sample measurements. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of. Fully tested with validations, warranty. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. The SpectraMax iD3 reader allows you to interact with your data any way you want, from anywhere you want. In . For readers without a cuvette port, simply check the box next to ‘PathCheck’. Step 2: Launch SoftMax Pro Software. The SpectraMax ABS Plus Microplate Reader is a compact and adaptive microplate reader capable of quantitating nucleic acid and protein samples in various formats such as cuvettes or microplates. Temperature control. Turn the reader off by pulling the power cord. The SpectraMax Mini Multi-Mode Microplate Reader performs your favorite applications including ELISA, DNA and protein quantitation, cell viability, gene expression with a user-friendly reader. NOTE: Limited support and literature is available for the SpectraMax 250 due to the senescence of the product. As a generous estimate, you might consider 300 W (at 120 Volts) per Spectramax reader, plus another 300 W for the Computer. When the incubator is on, the temperature may be controlled from 2°C above ambient to 60°C. Offer Ends In: 2 Days 10: 40: 19. For 40 years, we have partnered with scientists to help them achieve landmark discoveries. The SpectraMax® i3x Multi-Mode microplate reader measures spectral- based Absorbance, Fluorescence, and Luminescence on microplates that range from 6 to 384 wells. The SpectraMax® iD3 Multi Mode Microplate . The cuvette must be in place when you read the plate. Figure 3. Step 4: Incubate at room temperature for 1 hour, protected from light. Temperature control. Figure 2-23: Bottle holder To install the bottle holder: 1. Published Dec 16, 2020 | Updated May 19, 2023. View the Molecular Devices website. SAN JOSE, CA — Molecular Devices, LLC. Microplate reader settings. unconventional microplate reader assays, including imaging cytometry and western blots • Protocol sharing with a strong user community • Spectral optimization wizard to maximize assay signal window (SpectraMax i3x and Paradigm readers only) SoftMax Pro Software SoftMax Pro® Software is the most published0200-6262. The temperature of the microplate chamber can be regulated, if desired from 4°C above ambient to 45°C. Both readers feature an 8-channel read head for fast reads and a subset feature, the temperature independent PathCheck sensor that. Page 27 SpectraMax M2 and M2e Microplate Reader User Guide Place a standard 1 cm cuvette that contains the aqueous/solvent mixture you use for the plate samples into the cuvette port. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M5e Microplate Reader transforms each well in a microplate to a fixed. What is the minimum well volume needed to run a 384 well plate? Minimum volume depends on the solution (curved vs. The SpectraMax® ABS and ABS Plus absorbance microplate readers provide the flexibility, sensitivity, and convenience for a wide range of assays such as ELISAs, microbial growth, and protein. 2. The SpectraMax ® i3x Platform from Molecular Devices is a multi-mode microplate reader that evolves with your future needs and offers an unlimited breadth of application possibilities. Temperature in the microplate chamber is isothermal, both at ambient and when the incubator is turned on. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. No additional manuals are available. . The ABS Plus reader ranges from 190 to 1000 nm in wavelength which is equivalent to having 810 free filters. Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader This page contains the table of contents of resources available for the SpectraMax Microplate Reader Suite of Products. 0 CART STOREVersaMax Microplate Reader An affordable, tunable microplate reader for 96-well format Affordable The robot-compatible VersaMax reader is cost-effective—users never have to buy filters. 1. The microplate (containing standards) was incubated at 37ºC in the preheated SpectraMax ABS Plus reader for 10 minutes. The SpectraMax® i3x Multi-Mode microplate reader measures spectral- based Absorbance, Fluorescence, and Luminescence on microplates that range from 6 to 384 wells. Bookings are free, but you will need to use iLab to book. With three modes of detection for UV/Vis absorbance, fluorescence and luminescence, the SpectraMax® Mini reader is user-upgradeable - from two to three modes, providing researchers with a versatile instrument. Prerequisites: SoftMax Pro 7. Molecular Devices SpectraMax ® i3x MultiMode Microplate Reader, in combination with luminescent assays for cell viability and cytotoxicity, provides a sensitive and rapid way to measure the number of viable cells in culture and quantify the cytotoxic effects of experimental treatment. . 5 25 75 25 50 50 50 100 0 100 结合反应 Table 2. Detailed specifications of each model reader are reported in Section 7, “Specifications”. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and. microplate reader. Fluorescence microplate readers are multimode spectrophotometers with the capability to detect and measure fluorescence emitted by analytes in a multi-well sample format. . Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. microplates or in a cuvette at a. The plate reader optics are tunable for absorbance. . ATP standard curves read on SpectraMax microplate readers. The SpectraMax® Glo Steady-Luc™ Reporter Assay Kit provides a highly sensitive assay for the quan-titation of firefly luciferase expression in mammalian cells. Components The main components of SpectraMax and VersaMax readers described in this manual are: • Control panel • Microplate drawerThe Molecular Devices SpectraMax 384 Plus is a UV/Vis spectrophotometer and microplate reader combination for more reliable and fast data reporting with built-in cuvette port and microplate drawer. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. It offers the additional advantage of HTRF ® (homogeneous time-resolved fluorescence) certification, allowing. For more detailed information and technical support of Life Technologies assays, please call 1-800-955- 6288 and enter extension 40266 or email drugdiscoverytech@lifetech. 0 out of 5 $ 5,999. SpectraMax M2 and SpectraMax M2e readers can acquire absorbance as well as fluorescence data for samples by issuing a single read command in SoftMax® Pro. For more sample throughput, both readersSupplier: Molecular Devices. We would like to show you a description here but the site won’t allow us. Path Check Pathlength Measurement SpectraMax i3x. 0 out of 5 $ 23,995 $ 22,495. ; Page 2 2) All labor charges to repair the product for a period of one (1) year from the original date of delivery will be paid by Molecular Devices Corporation. following formula: LLD = 3*SD blank *Concentration/(RLU sample-RLU blank). ” Our featured SpectraMax® L Microplate Reader Sensitive luminometer with programmable injector options for 96- and 384-well microplates. Molecular Devices SpectraMax M5 User Manual (86 pages) Multi-Mode Microplate Readers. Fluorescence Microplate Reader Comparison Chart. Category 2 and 3 microplate readers use gradient heating as well.